The following sequence is used for Read 1 and Read 2 adapter trimming. CTGTCTCTTATACACATCT. l Illumina DNA PCR-Free Prep, Tagmentation Adapter Trimming. The following sequence includes two adapter sequences joined by a plus sign. When performing adapter trimming, the software independently assesses each adapter for trimming.. In our work each read is 250 bp long. Read 1 is called the "forward" read, or the R1, and Read 2 is the "reverse" or R2 read (R1 and R2 are used in the file names - see post on that). The Illumina system knows that a Read 1 and Read 2 belong to the same piece of DNA because they will be physically "read" off the same spot on the chip.

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Illumina Adapter Sequences. This document provides the nucleotide sequences that comprise Illumina oligonucleotides used in Illumina sequencing technologies. These sequences are provided for the sole purpose of understanding and publishing the results of your sequencing experiments.. They're added separately at the beginning of each sequencing read to start the synthesis reaction (ie. Read 1 primer at the start of read 1, read 2 primer at the start of read 2). The templates themselves are also single stranded at this point and anchored to the flow cell by the P5 and P7 Illumina adapters. The actual primers used in the PCR.